Apart from having a simpler structure, rabbit mAbs offer higher binding affinity (10 to 100 times more) and specificity than murine mAbs, and they are easier to humanize. These properties of rabbit mAbs make them useful in cases where antigens exhibit weak immunogenicity.

Rabbit antibodies can recognize various target epitopes in humans than rodent antibodies, which is of great importance in basic and preclinical studies. You can also navigate www.bosterbio.com/services/custom-rabbit-monoclonal-antibody-development-service to know more about rabbit monoclonal antibodies.

Production of rabbit mAbs

Rabbits are first injected with antigen to elicit a humoral immune response. Spleen cells were isolated from these immunized rabbits and fused with myeloma cells. After fusion, hybridoma cells were isolated, filtered, and cultured to produce the required antibodies.

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Various technologies used to produce rabbit mAbs are hybridoma technology, phage display technology, and other alternative methods.

Hybridoma Technology

While rabbit mAbs developed using this technology are valuable reagents for laboratory testing and diagnostic applications, the methodology is difficult.

Phase Display Technology

This method develops a rabbit antibody library in a single-chain variable format (scFv) as well as in an antigen-binding fragment (Fab) format. The Fab format has greater advantages due to its monomer properties, higher stability, and affinity. the scFv format tends to dimerize, trimerize, and tetramerize.

Alternative Method

High-performance DNA technology by mass spectrometry is another method used to generate rabbit mAbs and to analyze the immune antibody repertoire.